I installed the trial version of Ghost 15 on my new Vista 64 machine and after running the recommended backups I now have a Status Message on the Home tab stating

The backup destination on Seagate (E:\) is unavailable.

My system specs ...
ASUS P5Q-SE
E8200
2 X 500GB (C: drive is 7200.11, E: drive is 7200.12)
I had another drive installed that I replaced with the 7200.12, so I assume that is why Vista called it E: instead of D:

The drive is very much available, I can move to the Status tab and see my backups are happy and complete. I can browse the drive using Windows Explorer.
Any ideas?